Polymerase Chain Reaction:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Agarose Gel Electrophoresis:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Hood:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Software:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Sequencing:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Countercurrent Chromatography:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis ..
Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Immunostaining:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
Blocking Assay:Article Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Article Snippet: .. The PCR products were run on a 1 % agarose gel at 80 V. Gels were visualised using the Bio Rad Universal Hood II GelDoc system (Bio Rad Laboratories Inc., Hercules, CA, USA) and images were taken using Quantity One (version 4.6.3) software. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Gene Primer Sequence β-actin F: GGA ATC CTG TGG CAT CCA TGA AAC R: AAA ACG CAG CTC AGT AAC AGT CCG Oct 3/4 F: TCT TTC CAC CAG GCC CCC GGC TC R: TGC GGG CGG ACA TGG GGA GAT CC Nanog F: TCA AGG ACA GGT TTC AGA AGC A R: GCT GGG ATA CTC CAC TGG TG c-myc F: AAG TTT GAG GCA GCA GTT AAA ATT ATG GCT GAA R: TGA CCT AAC TCG AGG AGG AGC TGG AAT C Nestin F: TCT GGA AGT CAA CAG AGG TGG R: ACG GAG TCT TGT TCA CCT GC Brachyury F: CAT GTA CTC TTT CTT GCT GG R: GGT CTC GGG AAA GCA GTG GC Fox A2 F: TGG TCA CTG GGG ACA AGG GAA R: GCA ACA ACA GCA ATA GAG AAC Open in a separate window Primer sequences for genes used in pluripotency and differentiation capability analysis Immunostaining Cells on microcarriers were fixed using 4 % paraformaldehyde followed by permeabilization and blocking using 0.1 % Triton X and 2 % BSA in PBS, respectively. ..
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